| PAW Patrol: The Dino Movie (2025) (voice) |
| To End All War: Oppenheimer & the Atomic Bomb (2023) Self - Science Educator |
| Jurassic World Camp Cretaceous: Hidden Adventure (2022) Brimford (voice) |
| Bliss (2021) Chris |
| Mank (2020) Upton Sinclair |
| Happy Halloween, Scooby-Doo! (2020) Bill Nye (voice) |
| A Brief History of Time Travel (2019) Self |
| Behind the Curve (2018) Self |
| Night of Too Many Stars: America Unites for Autism Programs (2017) Self |
| Food Evolution (2017) Self |
| Bill Nye: Science Guy (2017) Self |
| The Mars Generation (2017) Self |
| The Fog Bowl (2016) Self |
| Star Trek: Inside the Roddenberry Vault (2016) Self |
| Fight For Space (2016) Self |
| Must See TV: An All Star Tribute to James Burrows (2016) Self |
| Bill Nye's Global Meltdown (2016) Self |
| Global Citizen Festival 2015 (2015) Self |
| Bill Nye the Science Guy Tackles DeflateGate (2015) Self |
| An Honest Liar (2014) Self |
| Uncensored Science: Bill Nye Debates Ken Ham (2014) Self |
| Safety Smart Science with Bill Nye the Science Guy: Electricity (2008) Self |
| Principal Takes a Holiday (1998) Science Teacher |
| How to Juggle & Other Matters of Life & Death (1990) Nebish |
| Disney's Safety Hits Volume 1: Bike Safety With Bill Nye The Science Guy & I'm No Fool On Wheels Self |
| Safety Smart Science with Bill Nye the Science Guy: Renewable Energy Self |